Gene: COL1A1
mRNA Accession: Z74615
Mutation:COL1A1.txt
Chromosome: chr 17

Sequence Walker  




IVS22-1G>T


Instruction: c48613888a; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48613892 -4 48613888 0.1 0.1 0.0 1.0 100 -1.9 -1.9 0.0
No change 48613851 37 48613888 1.5 1.5 0.0 1.0 100 -1.6 -1.6 0.0
No change 48613842 46 48613888 5.3 5.3 0.0 1.0 100 -0.8 -0.8 0.0
Decreased 48613888 0 48613888 7.0 -1.8 -8.7 -127.8 NA -0.4 -2.3 -1.9



IVS22+3A>G


Instruction: t48614010c; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48614033 -21 48614012 4.8 4.8 0.0 1.0 100 -0.9 -0.9 0.0
No change 48614013 -1 48614012 0.8 0.8 0.0 1.0 100 -1.8 -1.8 0.0



IVS25-1G>A


Instruction: c48612488t; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
Increased 48612490 -2 48612488 5.0 5.1 0.0 1.0 103.1 -0.8 -0.8 0.0
Decreased 48612488 0 48612488 13.8 6.3 -7.6 -191.1 0.5 1.1 -0.6 -1.7
Increased 48612487 1 48612488 0.0 8.2 8.2 286.2 28616.6 -1.9 -0.1 1.8
Increased 48612484 4 48612488 2.2 2.5 0.3 1.2 123.3 -1.4 -1.4 0.1



IVS26+1G>A


Instruction: c48612433t; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
Increased 48612409 24 48612433 2.0 2.4 0.4 1.3 130.5 -1.5 -1.4 0.1
No change 48612398 35 48612433 3.2 3.2 0.0 1.0 100 -1.2 -1.2 0.0
No change 48612396 37 48612433 3.8 3.8 0.0 1.0 100 -1.1 -1.1 0.0
No change 48612393 40 48612433 4.3 4.3 0.0 1.0 100 -1.0 -1.0 0.0
No change 48612388 45 48612433 3.1 3.1 0.0 1.0 100 -1.3 -1.3 0.0
No change 48612387 46 48612433 1.0 1.0 0.0 1.0 100 -1.7 -1.7 0.0
No change 48612384 49 48612433 1.4 1.4 0.0 1.0 100 -1.6 -1.6 0.0



IVS26+1G>T


Instruction: c48612433a; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
Increased 48612409 24 48612433 2.0 2.9 0.8 1.8 179.6 -1.5 -1.3 0.2
No change 48612398 35 48612433 3.2 3.2 0.0 1.0 100 -1.2 -1.2 0.0
No change 48612396 37 48612433 3.8 3.8 0.0 1.0 100 -1.1 -1.1 0.0
No change 48612393 40 48612433 4.3 4.3 0.0 1.0 100 -1.0 -1.0 0.0
No change 48612388 45 48612433 3.1 3.1 0.0 1.0 100 -1.3 -1.3 0.0
No change 48612387 46 48612433 1.0 1.0 0.0 1.0 100 -1.7 -1.7 0.0
No change 48612384 49 48612433 1.4 1.4 0.0 1.0 100 -1.6 -1.6 0.0
Increased 48612429 4 48612433 -2.0 1.1 3.1 2.2 219.3 -2.4 -1.7 0.7



IVS26-2A>C


Instruction: t48612292g; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
Decreased 48612294 -3 48612291 4.5 4.1 -0.5 -1.4 72.3 -0.9 -1.1 -0.1
Decreased 48612291 0 48612291 15.4 8.0 -7.4 -171.7 0.5 1.4 -0.2 -1.6
Increased 48612290 1 48612291 1.3 5.5 4.2 18.7 1870.1 -1.7 -0.7 0.9
Increased 48612281 10 48612291 -0.6 1.5 2.0 2.8 280.8 -2.1 -1.6 0.4
Increased 48612275 16 48612291 -0.2 1.0 1.2 2.0 196.5 -2.0 -1.7 0.3



IVS27-1G>C


Instruction: c48612134g; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48612159 -25 48612134 1.6 1.6 0.0 1.0 100 -1.6 -1.6 0.0
No change 48612150 -16 48612134 5.4 5.4 0.0 1.0 100 -0.8 -0.8 0.0
Decreased 48612134 0 48612134 8.8 1.6 -7.3 -156.4 0.6 -0.0 -1.6 -1.6



IVS41+4A>C


Instruction: t48608339g; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
Increased 48608316 26 48608342 1.7 2.2 0.5 1.4 143.5 -1.6 -1.4 0.1
No change 48608307 35 48608342 4.2 4.2 0.0 1.0 100 -1.0 -1.0 0.0



IVS47+1G>A


Instruction: c48606454t; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48606447 7 48606454 2.0 2.0 0.0 1.0 100 -1.5 -1.5 0.0
Increased 48606431 23 48606454 0.9 1.2 0.3 1.2 124.0 -1.7 -1.7 0.1
No change 48606426 28 48606454 0.9 0.9 0.0 1.0 100 -1.7 -1.7 0.0
No change 48606416 38 48606454 8.3 8.3 0.0 1.0 100 -0.1 -0.1 0.0
No change 48606407 47 48606454 2.2 2.2 0.0 1.0 100 -1.5 -1.5 0.0



IVS47-3C>G


Instruction: g48606365c; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48606368 -5 48606363 4.6 4.6 0.0 1.0 100 -0.9 -0.9 0.0
Decreased 48606363 0 48606363 15.2 9.3 -6.0 -62.7 1.5 1.4 0.1 -1.3
Decreased 48606362 1 48606363 0.5 0.3 -0.2 -1.2 86.5 -1.8 -1.9 -0.0
No change 48606335 28 48606363 3.1 3.1 0.0 1.0 100 -1.3 -1.3 0.0
No change 48606326 37 48606363 3.4 3.4 0.0 1.0 100 -1.2 -1.2 0.0
No change 48606316 47 48606363 10.3 10.3 0.0 1.0 100 0.3 0.3 0.0
Increased 48606366 -3 48606363 -0.2 1.7 1.9 3.3 333.4 -2.0 -1.6 0.4
Increased 48606365 -2 48606363 -1.4 5.9 7.3 59.0 5899.3 -2.2 -0.7 1.6



IVS48+1G>A


Instruction: c48606079t; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48606105 -26 48606079 3.1 3.1 0.0 1.0 100 -1.3 -1.3 0.0
No change 48606045 34 48606079 3.1 3.1 0.0 1.0 100 -1.3 -1.3 0.0
No change 48606040 39 48606079 4.3 4.3 0.0 1.0 100 -1.0 -1.0 0.0
No change 48606036 43 48606079 3.0 3.0 0.0 1.0 100 -1.3 -1.3 0.0
No change 48606031 48 48606079 10.0 10.0 0.0 1.0 100 0.2 0.2 0.0



IVS11+3-+6delGAGT


Instruction: d48616444,48616447; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
Increased 48616425 24 48616449 0.1 0.5 0.3 1.3 125.1 -1.9 -1.8 0.1
No change 48616416 33 48616449 0.7 0.7 0.0 1.0 100 -1.8 -1.8 0.0
No change 48616409 40 48616449 2.2 2.2 0.0 1.0 100 -1.5 -1.5 0.0



IVS12+1G>A


Instruction: c48616056t; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
Decreased 48616057 -1 48616056 1.3 0.4 -1.0 -1.9 51.5 -1.6 -1.9 -0.2
No change 48616016 40 48616056 6.9 6.9 0.0 1.0 100 -0.4 -0.4 0.0
No change 48616014 42 48616056 1.1 1.1 0.0 1.0 100 -1.7 -1.7 0.0



IVS13-10T>A


Instruction: a48615817t; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48615821 -13 48615808 3.1 3.1 0.0 1.0 100 -1.3 -1.3 0.0
Decreased 48615808 0 48615808 8.4 5.8 -2.6 -6.1 16.3 -0.1 -0.7 -0.6
Increased 48615816 -8 48615808 -0.3 7.1 7.4 140.1 14005.3 -2.0 -0.4 1.6



IVS14+5G>A


Instruction: c48615749t; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48615700 53 48615753 0.7 0.7 0.0 1.0 100 -1.8 -1.8 0.0



IVS15-33--44delCTGCCATCTCTT


Instruction: d48615661,48615672; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48615700 -60 48615640 0.7 0.7 0.0 1.0 100 -1.8 -1.8 0.0
Decreased 48615643 -3 48615640 3.1 2.4 -0.7 -1.7 59.7 -1.3 -1.4 -0.2
Increased 48615640 0 48615640 12.8 14.0 1.2 2.4 236.5 0.9 1.1 0.3



IVS16-2A>G


Instruction: t48615107c; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48615112 -6 48615106 2.4 2.4 0.0 1.0 100 -1.4 -1.4 0.0
Decreased 48615106 0 48615106 11.4 3.3 -8.2 -286.2 0.3 0.6 -1.2 -1.8
No change 48615069 37 48615106 0.6 0.6 0.0 1.0 100 -1.8 -1.8 0.0
No change 48615061 45 48615106 0.8 0.8 0.0 1.0 100 -1.8 -1.8 0.0
Increased 48615107 -1 48615106 -6.6 0.9 7.6 1.9 192.8 -3.4 -1.7 1.7
Increased 48615099 7 48615106 -0.1 0.2 0.3 1.2 116.4 -2.0 -1.9 0.1



IVS17+1G>C


Instruction: c48615006g; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48615024 -18 48615006 2.2 2.2 0.0 1.0 100 -1.5 -1.5 0.0
No change 48615015 -9 48615006 0.6 0.6 0.0 1.0 100 -1.8 -1.8 0.0
Increased 48614988 18 48615006 2.6 3.5 0.8 1.8 179.6 -1.4 -1.2 0.2
No change 48614966 40 48615006 0.4 0.4 0.0 1.0 100 -1.9 -1.9 0.0
No change 48614960 46 48615006 9.9 9.9 0.0 1.0 100 0.2 0.2 0.0
No change 48614958 48 48615006 2.8 2.8 0.0 1.0 100 -1.3 -1.3 0.0
Increased 48615002 4 48615006 -2.0 0.5 2.5 1.4 140.9 -2.4 -1.8 0.6



IVS18+1G>A


Instruction: c48614873t; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
Increased 48614851 22 48614873 1.3 1.8 0.4 1.4 135.5 -1.6 -1.5 0.1
No change 48614830 43 48614873 8.1 8.1 0.0 1.0 100 -0.2 -0.2 0.0
Decreased 48614858 15 48614873 0.1 -0.2 -0.2 -1.0 NA -1.9 -2.0 -0.0



IVS18-1G>A


Instruction: c48614771t; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48614800 -29 48614771 0.9 0.9 0.0 1.0 100 -1.7 -1.7 0.0
No change 48614795 -24 48614771 1.2 1.2 0.0 1.0 100 -1.7 -1.7 0.0
Increased 48614773 -2 48614771 2.2 2.2 0.0 1.0 103.1 -1.5 -1.5 0.0
Decreased 48614771 0 48614771 10.4 2.8 -7.6 -191.1 0.5 0.3 -1.3 -1.7
Increased 48614752 19 48614771 0.0 0.2 0.2 1.1 113.7 -1.9 -1.9 0.0
No change 48614734 37 48614771 2.5 2.5 0.0 1.0 100 -1.4 -1.4 0.0
No change 48614725 46 48614771 2.3 2.3 0.0 1.0 100 -1.4 -1.4 0.0
Increased 48614770 1 48614771 -2.6 5.6 8.2 47.7 4770.3 -2.5 -0.7 1.8



IVS19+1G>A


Instruction: c48614671t; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48614681 -10 48614671 3.6 3.6 0.0 1.0 100 -1.1 -1.1 0.0
No change 48614643 28 48614671 0.4 0.4 0.0 1.0 100 -1.8 -1.8 0.0
No change 48614638 33 48614671 2.6 2.6 0.0 1.0 100 -1.4 -1.4 0.0
No change 48614634 37 48614671 0.9 0.9 0.0 1.0 100 -1.8 -1.8 0.0
No change 48614632 39 48614671 0.2 0.2 0.0 1.0 100 -1.9 -1.9 0.0
No change 48614625 46 48614671 3.9 3.9 0.0 1.0 100 -1.1 -1.1 0.0



IVS19+1G>C


Instruction: c48614671g; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48614681 -10 48614671 3.6 3.6 0.0 1.0 100 -1.1 -1.1 0.0
No change 48614643 28 48614671 0.4 0.4 0.0 1.0 100 -1.8 -1.8 0.0
No change 48614638 33 48614671 2.6 2.6 0.0 1.0 100 -1.4 -1.4 0.0
No change 48614634 37 48614671 0.9 0.9 0.0 1.0 100 -1.8 -1.8 0.0
No change 48614632 39 48614671 0.2 0.2 0.0 1.0 100 -1.9 -1.9 0.0
No change 48614625 46 48614671 3.9 3.9 0.0 1.0 100 -1.1 -1.1 0.0



IVS20+2T>C


Instruction: a48614485g; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48614459 27 48614486 9.9 9.9 0.0 1.0 100 0.2 0.2 0.0
No change 48614454 32 48614486 7.0 7.0 0.0 1.0 100 -0.4 -0.4 0.0
No change 48614439 47 48614486 5.1 5.1 0.0 1.0 100 -0.8 -0.8 0.0



IVS20-2A>G


Instruction: t48614270c; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48614297 -28 48614269 4.5 4.5 0.0 1.0 100 -1.0 -1.0 0.0
No change 48614280 -11 48614269 1.2 1.2 0.0 1.0 100 -1.7 -1.7 0.0
No change 48614279 -10 48614269 0.8 0.8 0.0 1.0 100 -1.8 -1.8 0.0
Decreased 48614269 0 48614269 12.7 4.5 -8.2 -286.2 0.3 0.8 -0.9 -1.8
Decreased 48614250 19 48614269 3.5 3.2 -0.3 -1.2 81.8 -1.2 -1.2 -0.1
No change 48614232 37 48614269 0.4 0.4 0.0 1.0 100 -1.9 -1.9 0.0
No change 48614229 40 48614269 0.5 0.5 0.0 1.0 100 -1.8 -1.8 0.0
Increased 48614270 -1 48614269 -2.5 5.1 7.6 33.3 3333.6 -2.5 -0.8 1.7



IVS3+1G>C


Instruction: c48618995g; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48618966 29 48618995 0.0 0.0 0.0 1.0 100 -1.9 -1.9 0.0
No change 48618944 51 48618995 4.3 4.3 0.0 1.0 100 -1.0 -1.0 0.0



IVS5-1G>A


Instruction: c48617945t; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48617965 -20 48617945 0.6 0.6 0.0 1.0 100 -1.8 -1.8 0.0
No change 48617955 -10 48617945 0.2 0.2 0.0 1.0 100 -1.9 -1.9 0.0
Decreased 48617945 0 48617945 10.9 3.3 -7.6 -191.1 0.5 0.4 -1.2 -1.7
Decreased 48617930 15 48617945 7.4 7.1 -0.2 -1.2 85.8 -0.3 -0.4 -0.0
Increased 48617943 2 48617945 -0.2 1.5 1.7 2.9 292.8 -2.0 -1.6 0.4



IVS5-2A>G


Instruction: t48617946c; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
No change 48617965 -20 48617945 0.6 0.6 0.0 1.0 100 -1.8 -1.8 0.0
No change 48617955 -10 48617945 0.2 0.2 0.0 1.0 100 -1.9 -1.9 0.0
Decreased 48617945 0 48617945 10.9 2.7 -8.2 -286.2 0.3 0.4 -1.3 -1.8
Increased 48617930 15 48617945 7.4 7.4 0.0 1.0 103.1 -0.3 -0.3 0.0
Increased 48617946 -1 48617945 -3.1 4.5 7.6 22.2 2215.5 -2.6 -1.0 1.7



IVS8+1G>C


Instruction: c48617388g; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
Increased 48617370 18 48617388 5.6 6.4 0.8 1.8 179.6 -0.7 -0.5 0.2
No change 48617351 37 48617388 1.3 1.3 0.0 1.0 100 -1.6 -1.6 0.0
No change 48617343 45 48617388 3.1 3.1 0.0 1.0 100 -1.3 -1.3 0.0



IVS9-1G>T


Instruction: c48616674a; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
Decreased 48616674 0 48616674 14.2 5.5 -8.7 -430.0 0.2 1.2 -0.7 -1.9
Increased 48616673 1 48616674 2.7 3.4 0.7 1.7 166.6 -1.4 -1.2 0.2
Increased 48616667 7 48616674 0.2 2.1 2.0 3.9 391.8 -1.9 -1.5 0.4
Increased 48616672 2 48616674 -0.3 4.0 4.3 16.2 1620.7 -2.0 -1.1 0.9



c.1299+1G>C


Instruction: c48614873g; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
Increased 48614858 15 48614873 0.1 1.2 1.1 2.2 216.5 -1.9 -1.7 0.2
Increased 48614851 22 48614873 1.3 2.3 0.9 1.9 192.0 -1.6 -1.4 0.2
No change 48614830 43 48614873 8.1 8.1 0.0 1.0 100 -0.2 -0.2 0.0



c.333G>T;.c333+1G>C


Instruction: c48619277a.c48619192g; Sequence Walker
Type of changeGenomic CoordinatePosition Relative
to Natural Site
Closest
Natural Site
Initial(Ri)Final(Ri)Δ RiFold change% Binding (Final/Initial)Initial(Z)Final(z)Δ Z
Decreased 48619193 -1 48619192 4.9 3.0 -1.9 -3.8 26.5 -0.9 -1.3 -0.4

Legend:    Site Abolished   Leaky Site   Cryptic Site created   Strengthened Pre-existing site   Pre-existing Site   Weakened Pre-existing Site